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Purification and characterization of carbonic anhydrase from sheep kidney and effects of sulfonamides on enzyme activity

Demirdag, Ramazan; Comakli, Veysel; Senturk, Murat; Ekinci, Deniz; Kufrevioglu, O. Irfan; Supuran, Claudiu T. (Pergamon-Elsevier Science Ltd, 2013)

Carbonic anhydrase (CA, EC: 4.2.1.1) was purified from sheep kidney by affinity chromatography on a Sepharose 4B-tyrosine-sulfanilamide column. By means of two consecutive procedures, the enzyme (sCA) was purified 227.61-fold ...