Yazar "Genc, Oktay" için listeleme
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Bi-antigenic immunoassay models based on the recombinant PvpA proteins for Mycoplasma gallisepticum diagnosis in chickens
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Amer Assoc Veterinary Laboratory Diagnosticians Inc, 2010)The present study aimed to produce the relatively conserved central fragment of the Mycoplasma gallisepticum PvpA cytadhesin as recombinant antigen and to determine its species-specific diagnostic potential in comparison ... -
Detection of Brucella Antibody and DNA in Cow Milk by ELISA and PCR Methods
Terzi, Goknur; Buyuktanir, Ozlem; Genc, Oktay; Gucukoglu, Ali; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)The aim of the present study was to determine comparatively the presence of anti-Brucella antibody and Brucella DNA in cow milk. Anti-Brucella antibody was detected by ELISA based on the lipopolysaccharide (LPS) as diagnostic ... -
Determination of Clostridium perfringens toxin-types in sheep with suspected enterotoxemia in Kars province, Turkey
Goekce, Halil Ibrahim; Genc, Oktay; Soezmen, Mahmut; Goekce, Gurbuz (Scientific Technical Research Council Turkey-Tubitak, 2007)The study was designed to determine the types of Clostridium perfringens and their toxins in sheep with suspected enterotoxemia in Kars province. For this purpose, 260 dead sheep with suspected enterotoxemia and 35 clinically ... -
Development and validation of sandwich quantitative ELISA prototype based on the bovine IFNg for the detection of cellular immunity
Genc, Oktay; Buyuktanir, Ozlem; Serdar, Gulnur; Kilicoglu, Yunus; Otlu, Salih (Scientific Technical Research Council Turkey-Tubitak, 2015)Immunodiagnostic tests based on in vitro enzymatic methods are mostly preferred to in vivo methods for the detection of cellular immunity due to their cost, applicability, repeatability, and higher sensitivity. Therefore, ... -
Development of an individual rapid test based on enzymatic immunofiltration assay for detection of anti-Brucella abortus antibody in bovine sera
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Sage Publications Inc, 2011)To detect bovine antibody directed to smooth lipopolysaccharide (LPS), cell lysate (LYS), O-polysaccharide (OPS), and LPS-deprived chromatographic fractions (ChF) of Brucella abortus, 2 bi-antigenic diagnostic models based ... -
Development of Qualitative and Quantitative ELISA Models for Bovine Brucellosis Diagnosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010)In this study, a qualitative and a quantitative ELISA models (iELISA and qELISA, respectively) have been developed for screening and quantifying IgG isotype anti-Brucella abortus LPS antibody. For developing high sensitive ... -
The Effects of High and Low Altitudes on Conjunctival Flora in Sport and Work Horses: A Field Study in the Northeast Anatolia Region of Turkey (Kars and Igdir)
Baran, Vedat; Ozaydin, Isa; Genc, Oktay; Otlu, Salih; Yayla, Sadik; Kilic, Engin; Saglam, Aliye Gulmez (Kafkas Univ, Veteriner Fakultesi Dergisi, 2015)The purpose of this study was to identify the conjunctival microflora in healthy horses living at various altitudes and used for both sports as well as work activities, and determine the predispositions they may cause in ... -
Evaluation of Total Oxidant/Antioxidant Capacity and Ceruloplasmin Levels in Horses with Inflammatory Airway Disease
Arslan, Handan Hilal; Genc, Oktay; Cenesiz, Metin; Arslan, Ali; Genc, Evrim; Cenesiz, Sena (Univ Fed Rio Grande Do Sul, 2017)Background: Respiratory disease negatively affects the physiological performance of racehorses. Inflammatory Airway Disease (IAD) is a typical respiratory disease that affects young horses. The pathogenesis of IAD remains ... -
Investigation of bovine brucellosis in the Northeastern Turkey
Sahin, Mitat; Genc, Oktay; Uenver, Ahmet; Otlu, Salih (Springer, 2008)Bovine brucellosis, caused by Brucella abortus, is a significant problem for both public and animal health in Turkey. This study was conducted on the calving seasons between 2001 and 2006. A total of 626 serum samples of ... -
Molecular and serological evidence of Anaplasma phagocytophilum infection of farm animals in the Black Sea Region of Turkey
Gokce, Halil Ibrahim; Genc, Oktay; Akca, Atila; Vatansever, Zati; Unver, Ahmet; Erdogan, Hidayet Metin (Akademiai Kiado Zrt, 2008)This study was designed to determine the presence and the prevalence of Anaplasma phagocytophilum infection in sheep and cattle in the Middle and Eastern Black Sea Regions of Turkey in which the potential vector, Ixodes ... -
Monitoring of the immune response to B-abortus S19 conjunctival vaccine in cattle
Genc, Oktay; Serdar, Gulnur; Buyuktanir Yas, Ozlem; Genc, Evrim (Ankara Univ Press, 2019)Brucella abortus S19strain is one of the most preferred strains in vaccines against brucellosis in cattle. However, monitoring of the B. abortus S19 vaccine is difficult due to non availability of sustainable immunoreactive ... -
Production, Purification and Characterization of the Recombinant Brucella abortus rP17 Protein
Buyuktanir, Ozlem; Genc, Oktay; Yurdusev, Nevzat (Kafkas Univ, Veteriner Fakultesi Dergisi, 2011)Immunoreactive cytosolic P17 protein of Brucella abortus was produced in Escherichia coli as 6xHistidine tagged recombinant protein (rP17) by cloning the p17 gene into pColdI cold-shock expression vector. DNA sequence ... -
Rapid Immunofiltration Assay As A Field Diagnostic Tool For Ovine Brucellosis
Buyuktanir, Ozlem; Genc, Oktay; Celebi, Ozgur; Yurdusev, Nevzat (Taylor & Francis Inc, 2012)This work describes the development of two rapid immunofiltration assays, enzymatic (ERIFA) and non-enzymatic (NERIFA), for the rapid detection of ovine anti-Brucella antibodies. Brucella abortus lipopolysaccharide and ... -
Rapid immunofiltration assay based on colloidal gold-protein G conjugate as an alternative screening test for bovine and ovine brucellosis
Genc, Oktay; Buyuktanir, Ozlem; Yurdusev, Nevzat (Springer, 2012)A non-enzymatic rapid immunofiltration assay (NERIFA) was developed as an alternative field test for rapid detection of anti-Brucella antibody in bovine and ovine sera. The assay was based on Brucella abortus lipopolysaccharide ... -
A recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken Mycoplasma gallisepticum infections
Bueyuektanir, Ozlem; Yildirim, Tuba; Yakicier, Cengiz; Genc, Oktay; Yurdusev, Nevzat (Elsevier Science Bv, 2008)Mycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and ... -
Seroprevalance of Chlamydophila abortus in aborting ewes and dairy cattle in the north-east part of Turkey
Gokce, Halil Ibrahim; Kacar, Cihan; Genc, Oktay; Sozmen, Mahmut (Natl Veterinary Research Inst, 2007)The aim of the study was to determine the seroprevalance of Chlamydophila abortus in aborting ewes and cattle in the Kars province in the north-eastern part of Turkey. Thirty sheep flocks and 26 herds of dairy cattle out ...