Shimazuella alba sp. nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007

Abstract

A novel, Gram-stain-positive bacterium, designated KC615(T), was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615(T) formed a monophyletic clade with Shimazuella kribbensis KCTC 9933(T), sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine. The predominant menaquinones (> 10%) were MK-9(H-4) and MK-10(H-4). Major fatty acids were anteiso-C-15:0, C-20:0 and C-18:0. The genomic DNA G + C content observed for strain KC615(T) was 38.5 mol%. Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615(T) is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp. nov. is proposed. The type strain is KC615(T) (= JCM 33532(T) = CGMCC 4.7616(T)).