Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp. nov., Jiangella aurantiaca sp. nov. and Jiangella ureilytica sp. nov

Abstract

Three isolates, 5K138(T), 8K307T and KC60(T), with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliations of the strains. The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as LL-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H-4) as a major menaquinone. Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237(T), Jiangella rhizosphaerae NEAU-YY265(T) and Jiangella mangrovi 3SM4-07(T) with higher than 99% sequence identities. However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella. Therefore, strains 5K138(T), 8K307(T) and KC603(T) should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp. nov., Jiangella aurantiaca sp. nov. and Jiangella ureilytica sp. nov. are proposed, respectively. The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138(T) (=JCM 33518(T) =CGMCC 4.7672(T)), Jiangella aurantiaca 8K307(T) (=JCM 33519(T) =CGMCC 4.7621(T)) and Jiangella ureilytica KC603(T) (=JCM 33520(T) =CGMCC 4.7618(T)).