Jiangella anatolica sp. nov. isolated from coastal lake soil

Abstract

A novel actinobacterial strain, designated GTF31(T), was isolated from a coastal soil sample of Golcuk Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835(T) (99.4%), Jiangella alba DSM 45237(T) (99.3%) and Jiangella muralis DSM 45357(T) (99.2%). Optimal growth was observed at 28 degrees C and pH 7-8. Whole cell hydrolysates were found to contain LL-DAP, glucose, mannose, rhamnose and ribose. The predominant menaquinone was identified as MK-9(H-4). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycophospholipids and unidentified phospholipids. The major fatty acids were identified as anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0. The G+C content of the type strain was determined to be 72.5% and the size of the draft genome is 7.0Mb. The calculated digital DDH values between strain GTF31(T) and the type strains of J. gansuensis, J. alba, J. muralis and Jiangella alkaliphila ranged from 24.4 to 34.4% and ANI values ranged between 81.0 and 87.9%. Based upon the consensus of phenotypic and phylogenetic analyses as well as whole genome comparisons, strain GTF31(T) (=DSM 100984(T)=CECT 9378(T)) is proposed to represent the type strain of a novel species, Jiangella anatolica sp. nov.