GENETIC DIVERSITY OF TURKISH Lathyrus L. LANDRACES USING ISSR MARKERS

Abstract

Fifty-one Lathyrus sativus L. landraces and one L. clymenum L. landrace collected from Turkey and one L. sativus cultivar, Gurbuz, were evaluated with ISSR markers in this study to molecular characterization. Three ISSR primers were used and 45 DNA fragment were evaluated, of which 44 bands were polymorphic. The frequencies of scored bands ranged from 0.009 to 0.888 and averaged 0.363. The genotypes MLT04 and NEVO2 had high similarity with 0.825 according to pairwise grouping. The furthest pairwise group was Gurbuz and MLT02 with 0.244. The nearest genotype to Gurbuz was CNR03 with 0.577. The pairwise genetic distance between L. clymenum and L. sativus ranged from 0.353 (accession NEV01) to 0.637 (accession DEN04) and pairwise genetic distance to the cultivar Gurbuz was 0.375. Assessment of genetic relationships among Lathyrus genotypes made two main groups. One of them covered only Gurbuz variety and the other covered 52 Lathyrus landrace. L. sativus and L. clymenum separated prominently in the second group.