Effect of Local Puerarin Administration on Xenograft

Abstract

Aim: The purpose of this study was to investigate the healing potential of local puerarin administration on xenograft in cranial bone defect treatment in rats. Material and Method: A critical-size defect of 5-mm diameter was created in the calvarium of twenty-four rats. The animals were divided into three groups: group C (passive control), group XBG (treated with Bio-Oss), and group P-XBG (treated with puerarin + Bio-Oss combination). All animals were euthanized at 28 days postoperative. Stereologic analyses were performed. New bone area and connective tissue volumes were measured. Results: Mean new bone volumes were 1.57 +/- 0.13mm3, 1.24 +/- 0.10 mm3, and 0.31 +/- 0.09 mm3 in groups P-XBG, XBG and C respectively. The new bone volumes were statistically significant in groups P-XBG and XBG compared to group C (p <= 0.05). Moreover, the new bone formation in group P-XBG was statistically higher when compared to the XBG group (P <0.05). In the P-XBG group, mean connective tissue volume was 2.00 +/- 0.12 mm3. The connective tissue volumes were statistically higher in groups P-XBG and XBG compared to group C (p<0.05). The difference between group P-XBG and group XBG was also statistically significant (p<0.05). Discussion: Puerarin had a positive effect on xenograft and increased new bone formation.