EVALUATION OF AN SSR MARKER FOR MARKER-ASSISTED SELECTION IN KALE (BRASSICA OLERACEA var. ACEPHALA)

Abstract

SARIKAMIS, G., R. YANMAZ, A. BALKAYA and B. BELGE, 2010. Evaluation of an SSR marker for marker-assisted selection in kale (Brassica oleracea var. acephala). Bulg. J. Agric. Sci., 16: 36-41 The objective of the current study is to test an SSR marker (Ol12FO2) associated with methylsulphinylalkyl glucosinolates and its potential application for marker assisted selection (MAS) in kale (Brassica oleracea var. acephala). Methylsulphinylalkyl glucosinolates such as glucoiberin and glucoraphanin are known to have health promoting properties and to protect against various forms of cancer. Markers associated with such important traits are important to expedite breeding programs aimed to select for desired traits for further use in generating improved novel lines. The findings of the present study revealed that successful amplifications with genomic DNA from kale genotypes were observed with the SSR marker Ol12FO2 and bands of expected sizes were obtained. It was determined that Ol12FO2 was polymorphic among kale genotypes. The results obtained in the present study demonstrated successful amplification of markers within different vegetable brassicas.