The comparison of agglutination, hemagglutination inhibition and indirect hemagglutination tests in the serological diagnosis ofinfectious coryza in chickens

Abstract

This study was carried out to investigate the feasibility of agglutination, hemagglutination inhibition (HI) and indirect hemagglutination (IHA) tests in the diagnosis of Infectious Coryza in chickens and to compare these tests. The agglutination tests were performed by using both serotype specific plate agglutination test antigens (serotype A, B and C specific) and the whole cell plate agglutination test antigen that prepared from serotype C (Modesto) strain. 5 10 sera were tested in this study. 42.75% of the field sera were found positive and 57.25% were found negative in plate agglutination tests performed with whole cell antigen. The test sera of 31.37% were found positive in regards to have serotype A antibodies and 15.1% of them were found positive in regards to have serotype C antibodies and remaining 53.53% were found to be negative in plate agglutination test performed with serotype spesific plate agglutination antigens. Hemagglutination did not occur in hemagglutination test performed with fresh chicken erythrocytes. Whereas in hem agglutination test which was performed with glutaraldehyde-fixed chicken erythrocytes, 1/64 HA titer was determined. Consequently, while the agglutination tests performed with whole cell antigen was found to be sensitive, HI and IHA were inefficient in the detection of seropositive chickens. Plate agglutination test performed with serotype spesific antigens were found to be useful to determine chickens infected with these serotype strains.