The Effects of the Mosquito Repellent Liquids Containing Esbiothrin on the Nasal Mucosa of Rats in an Experimental Model

Abstract

Objective: We investigated the histopathological changes in the nasal mucosa of rats due to commonly used mosquito repellent liquids containing esbiothrin used to avoid the harmful effects of the mosquitoes, which requires exposure by inhalation. Material and Methods: In the study, 50 Sprague-Dawley type rats were used. Test group rats were exposed to 3% esbiothrin containing liquid by inhalation for 8 hours per day. Four test groups were formed by randomly chosen 10 rats, each after 1, 4, 8 and 12 weeks of exposure. Ten rats that were kept away from esbiothrin formed the control group. All rats were sacrificed. Mucosa samples of the inferior concha were obtained and were stained with hematoxylene and eosin (H&E). The samples were histopathologically examined for evidence of inflammation and intraepithelial inflammatory cells, increase in the number of goblet cells and mucoid glands, loss of cilia and epithelium and existence of metaplasia. Results: Comparison of the test and control groups revealed significant differences in the presence of inflammation and intraepithelial inflammatory cells, increase in the number of goblet cells and mucoid glands, loss of cilia and epithelium. However, there was no significant difference in the existence of metaplasia between the two groups. Conclusion: In conclusion, it appears that the histopathological changes in the nasal mucosa caused by commonly used esbiothrin, which is thought to be harmless, shows similar findings with toxic rhinitis. Further investigation on humans supported by clinical and laboratory data may be suitable.