Regional brain and sex differences in the plasma progesterone concentration of sheep

Abstract

Progesterone concentration was measured in specific brain regions of the ram and anestrous ewe to provide reference values for future studies to investigate if local CNS lesions resulting from neurodegenerative diseases of sheep are associated with progesterone loss. Using radioimmunoassay, plasma progesterone was recorded throughout all brain regions assayed. No significant differences were found between the ewe and ram for any brain regions. There were however, significant differences between the different regions of an individual brain (P < 0.05). Plasma progesterome concentration for the highest to the lowest value recorded was as follows: 2.93 +/- 0.85 and 2.77 +/- 0.51 ng/g in the frontal cortex; 2.33 +/- 0.67 and 2.25 +/- 0.48 ng/g in the parietal cortex; 1.32 +/- 0.36 and 1.29 +/- 0.35 ng/g in the temporal cortex; 1.25 +/- 0.32 and 1.25 +/- 0.31 ng/g in the occipital cortex; 1.24 +/- 0.30 and 1.23 +/- 0.31 ng/g in the corpus callosum; 1.16 +/- 0.30 and 1.21 +/- 10.38 ng/g in the cerebellum; 1.09 +/- 0.30 and 1.12 +/- 0.39 ng/g in the medulla oblongata of the ewe and ram, respectively. Plasma progesterone concentration in the ewe (0.28 +/- 0.06 ng/ml) was significantly higher than that in the ram (0.10 +/- 0.03 ng/ml) (P < 0.001). Furthermore, plasma progesterone concentration in all sheep was several times lower than that of any regions of the brain. The results indicate that the sheep brain accumulates progesterone in significant levels, which may be independent of the circulating progesterone. The brain progesterone concentration in CNS regions assayed was similar for the ram and anestrous ewe. Neurodegenerative processes in visna, border disease and enzootic ataxia should be questioned in further studies if they are associated with local progesterone loss. (C) 2006 Elsevier B.V. All rights reserved.