Comparative examination of drug susceptibility of Mycobacterium tuberculosis by proportion method on Löwenstein-Jensen medium and middlebrook 7H10 agar

Abstract

In this study, the antibiotic susceptibilities of 50 Mycobacterium tuberculosis strains against isoniazide, rifampicin, streptomycin and ethambutol were investigated by proportion method on both Löwenstein-Jensen (LJ) medium and Middlebrook 7H10 agar. The concordance between two media was assessed by McNemar statistical test, and the accordance of the media used for isoniazide, rifampicin, streptomycin and ethambutol susceptibilities was found to be 88%, 74%, 68% and 90%, respectively. The differences between the media for the detection of resistance to streptomycin and rifampicin, were found to be statistically significant (p<0.001). According to the susceptibility test results using proportion method, resistance to antituberculosis drugs obtained from LJ medium was higher than that of Middlebrook 7H10 agar.