Praziquantel-dymethylsuifoxide solution: The side effect on hepato-biliary system (an experimental study)

Abstract

Aim In this experimental study, the side effects of praziquantel and dymethylsulfoxide on the hepatobiliary system has been investigated comparing with alcohol, hypertonic and normal saline. Material and Methods This study contains five groups of ten rats each. In all groups, transduodenal choledochal cannulation was done and either dymethylsulfoxide, praziquantel in dymethylsulfoxide solution, 0.9% NaCI, 20% NaCI or 98% alcohol were injected. Before surgery, blood samples were taken for measuring AST, ALT, ALP, GGT levels, and repeated every months for three months period. At the end of study, all rats were sacrificed; hepatobiliary excision was done. Results Biochemical content and blood samples have not statistically significant difference at the beginning of the study. After protoscolocidals injection into common bile duct, ALT, AST and ALP levels were siginificantly found higher at hypertonic saline group than control's (p<0.05). GGT level in hypertonic saline group was similar to control's. Also allthe biochemical results were siginificantly higher at dymethylsulfoxide, praziquantel in dymethylsulfoxide solution and alcohol groups than hypertonic saline and control groups (p<0.05). Although ALT, AST and ALP levels were found highest in hypertonic saline group; GGT level were was highest in dymethylsulfoxide group, (p<0.05) (Figure 1, 2, 3, 4). Histopathological research has shown that hepatoceluler changes were siginificantly higher in hypertonic saline group than control group, (p<0.05) Also it was higher in alcohol, dymethylsulfoxide and praziquantel in dymethylsulfoxide solution groups than the others, (p<0.05) Although dymethylsulfoxide and hypertonic saline have similar side effects on biliary tract; praziquantel in dymethylsulfoxide solution solution has stronger side effect then them, (p<0.05) alcohol has strongest side effect on biliary tract, (p<0.05). Conclusions As a conclusion, it is thought that praziquantel in dymethylsulfoxide solution solution has similar risk to alcohol and more than hypertonic saline on hepatobiliary tract in intraoperative use for hepatic hydatidosis.