Comparison of aspirin's antioxidant effect with selenium and vitamin E by measuring mitochondrial selenium dependent glutathione peroxidase and cytosolic total glutathione peroxidase activity in mice liver

Abstract

In this study, the antioxidant effect of aspirin was studied and compared with selenium and vitamin E on the activities of mitochondrial selenium dependent glutathione peroxidase (Se-GPX) and cytosolic total glutathione peroxidase (t-GPX). After the injection of selenium, vitamin E and aspirin intraperitonally to the mice, they were killed by servical dislocation at the 2, 4, 8, 12 and 24 h and their livers were removed. Enzyme fractions were obtained from livers. Mitochondrial fractions were used to find out the changes in the Se-GPX activities and cytosolic fractions were used to find out the changes in the total GPX activity. Aspirin increased cytosolic t-GPX activity in 12 and 24 h and mitochondrial Se-GPX activity in 8 h when compared with controls (p < 0.05). The t-GPX activity was less than vitamin E in 2 h and selenium in 12 h (p < 0.05). The study shows that aspirin has an antioxidant effect by increasing the cytosolic t-GPX and mitochondrial SeGPX activity. The antioxidant effect of aspirin is less than selenium and vitamin E.