Actinoplanes abujensis sp nov., isolated from Nigerian arid soil

Abstract

A novel actinobacterial strain, A4029(T), isolated from arid soil of Abuja, Nigeria, and provisionally assigned to the genus Actinoplanes, was subjected to a polyphasic taxonomic study. 16S rRNA gene sequence similarity studies showed that strain A4029(T) belonged to the genus Actinoplanes, being most closely related to Actinoplanes brasiliensis DSM 43805(T) (98.9%) and Actinoplanes deccanensis DSM 43806(T) (98.0%); similarity to other type strains of the genus Actinoplanes ranged from 96.2 to 97.9%. Chemotaxonomic data [major menaquinone MK-9(H-4); major polar lipids phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol; characteristic sugars arabinose and xylose; major fatty acids iso-C-15:0, anteiso-C-15:0, iso-C-16:0, C-17:1 omega 9c and iso-C-14:0] confirmed the affiliation of strain A4029(T) to the genus Actinoplanes. The results of DNA DNA hybridizations and phylogenetic analysis, together with phenotypic and biochemical test data, allowed strain A4029(T) to be differentiated from strains of other Actinoplanes species. Therefore, strain A4029(T) represents a novel species, for which the name Actinoplanes abujensis sp. nov. is proposed, with A4029(T) (=DSM 45518(T)=NRRL B-24835(T)=KCTC 19984(T)) as the type strain.