Lack of evidence for association between endothelial nitric oxide synthase gene polymorphisms (T-786C AND G894T) and early-onset coronary artery disease

Abstract

In this study, we investigated the association of two genetic variants of the endothelial nitric oxide synthase (eNOS), T786C and G894T, with CAD. Polymerase chain reaction and restriction fragment length polymorphism analyses were performed to detect the variants of eNOS gene in 102 patients with coronary artery disease and 103 healthy controls under the age 55. The statistical analysis was done by Chi square. The genotype and allele frequencies of CAD patients and controls were compared for the T786C genetic variant of eNOS. No evidence of significant difference was found between the two groups in the frequencies of the genotype [p = 0.810, OR = 0.77(0.25-2.15)] and allele [p = 0.173, OR = 0.72(0.47-1.11)]. In addition, the genotype and allele frequencies of CAD patients and controls were compared for the G894T genetic variant of eNOS. Similarly, no evidence of significant difference were found between the frequencies of genotype [p = 0.627, OR = 0.69 (0.25-1.88)] and allele [p = 0.610, OR = 0.87 (0.56-1.36)]. The present study provides evidence that the T786C and G894T polymorphism of the eNOS gene are not associated with the early-onset coronary artery disease in our population. © 2007 Asian Network for Scientific Information.