Identification and evaluation of clinically significant Nocardia brasiliensis, Nocardia farcinica and Nocardia otitidiscaviarum strains using pyrolysis mass spectrometry (PyMS)

Abstract

Out of a total of thirty-nine Nocardia strains, eight species of N. brasiliensis, seventeen species of N. farcinica and fourteen species of N. otitidiscaviarum were identified using Pyrolysis mass spectrometry. N428, N477 N. brasiliensis; N669, N233 N. farcinica; and N231, N232 N. otitidiscaviarum duplicated strains were clustered in their own groups. Strains belonging to Nocardia brasiliensis, Nocardia farcinica and Nocardia otitidiscaviarum formed distinct pyrogroups corresponding to clusters defined in the numerical study. It can be concluded that PyMS provides a rapid and reproducible method of evaluating the taxonomic significance of Nocadia species.